Media and Growth Conditions Biosurfactant production was carried out in a 3 L laboratory fermentor with working volume of 2. Mass spectrum of biosurfactant produced by P. Then pH adjusted to 8. This bacterium produces rhamnolipids as secondary metabolite and their production coincides with the stationary growth phase [ 65 , 95 , ]. Bioreactor is often applied in continuous or fed-batch fermentation in biosurfactant production. Pure paraffin oil and cottonseed oil were used as substrates for the production of surfactin using Bacillus subtilis Joshi et al. Further purification, structural characterization of biosurfactant and genetic regulation of biosurfactant production are in progress.

Nowadays, with current advances in biotechnology and due to the increasing environmental awareness, attention has shifted to the alternative environmental friendly process for production of different types of biosurfactants from bio-based resources as microorganisms [ 4 , 5 ]. Enhancement of Bacillus subtilis lipopeptide biosurfactants production through optimization of medium composition and adequate control of aeration. Metabolism and gene regulation of rhamnolipid production Biosurfactants are considered ideal for environmental application, due to their numerous advantages over their chemical counterparts, such as biodegradability and less toxicity [ 60 ]. Emulsification index of supernatants obtained with sugar cane molasses or glycerol as substrates. The first of them is the QscR factor. The production is repressed in exponential phase and low cell density while is activated in stationary phase and high cell densities.

biosurfactant production thesis

In order to complete the nature of biosurfactants produced by the three lactobacilli strains, the presence of phosphates in their composition was sought.

Introduction Great emphasis producction recently been given to the environmental impacts caused by chemical surfactants due to their toxicity and difficulty in being degraded in the environment [ 1 ]. Evidence for amino acid activating enzymes. Subscribe to Table of Contents Alerts.

However, a wide variety of roles for biosurfactants have been described including their antimicrobial [ 9 — 12 ], antiadhesive [ 1314 ], emulsifying [ 512 ], and antioxidant properties [ 15 ]. Bioreactor is often applied in continuous biosutfactant fed-batch fermentation in biosurfactant production.


It can be observed from Figures 3 a and 3 b that surface tension decreases producfion when the concentration of crude biosurfactants increases. Surface-active agents from two Bacillus species.

BioMed Research International

Results obtained in biosurfactant production with waste motor lubricant oil and peanut oil cake suggested the possibility of industrial production of biosurfactants using economically cheaper carbon sources. The global biosurfactants market volume is expected to reachView at Google Scholar G.

biosurfactant production thesis

Utilization of restaurants waste oil as a precursor for sophorolipid production. Emulsifying activity of biosurfactants produced by the three Lactobacillus strains while using sugar cane molasse and glycerol as substrate. Bioavailability of contaminants in soil to the metabolizing organisms is influenced by factors such as desorption, diffusion and dissolution.

The effects of bioremediation on biosurafctant oil degradation in oil polluted environments.

The result was considered positive for biosurfactants production when the drop was flat and the cultures that gave rounded drops were scored as negative, indicative of the lack of biosurfactant production [ 37 ]. Stationary phase and quorum sensing-related factors Stationary phase is a physiological state frequently related to nutrient scarcity.

In relation to factors that inhibit the rhamnolipid production in the exponential phase, two regulators can be identified. Media and Growth Conditions Biosuurfactant production was carried out in a 3 L laboratory fermentor with working volume of 2.

Biosurfactant Production by Pseudomonas aeruginosa from Renewable Resources

Introduction The surface-active compounds commonly used in many industries are chemically synthesized; they are widely used in almost every sector of recent industry Samadi et al. Biosurfactants produced with all the three strains while using glycerol as substrate are mainly composed of sugars and lipids. Pure paraffin oil and cottonseed oil were used as substrates for the production of surfactin using Bacillus subtilis Joshi et al.

J Am Oil Chem Soc.

High hydrophobicity and solid-water distribution ratios of some pollutants result in their interaction with non-aqueous phases and soil organic matter. They are very important chemical compounds which are used in a variety of products with very high volume because of their domestic and industrial applications [ 23 ]. Biosurfactant production by Bacillus subtilis using cassava processing effluent.


Bacterial biosurfactants were initially proposed to function as emulsifiers of biosurvactant hydrocarbons [ 8 ]. In addition, the downstream cost, low productivity and intense foaming formation during the biosurfactant production currently is a barrier for an economically viable production of biosurfactant [ 36 ].

Biosurfactants: Production and Applications

The third group represented by the inhibition diameter was slightly correlated with yield of biosurfactants. In the present work, waste frying oil was the best carbon source for biosurfactant synthesis. In this respect the feasibility to reusing old frying oil of sunflower for production rhamnolipids and sophorolipids, their use that lead to solve many ecological and industrial problems.

The experiment was conducted with four different sets— i bacterial cells alone, ii with fertilizer Urea and Dipotassium Phosphate 0.

The emulsifying activity observed could be due to the fact that adsorption of biosurfactants at the interface between water and oil allows a decrease in energy required to generate interfacial area, thus facilitating obtainment of drops with small diameter during emulsification step and allowing the formation of emulsions.

RpoS levels increase at the onset of stationary phase and in response to nutrient deprivation, even at low cell densities [ 91 ]. Biosurfctant expected, addition of surfactants inhibited the phenanthrene degradation by B-UM, and similar effect were found with addition of Triton X to Arthrobacte r sp.

MEL has also been shown to induce growth arrest, apoptosis and differentiation of mouse malignant melanoma cell cultures [ 5253 ].

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